Molekularbiologische und biochemische Untersuchungen von Hydroxycinnamoyltransferasen aus Coleus blumei und Glechoma hederacea

Hydroxyzimtsäureester sind bedeutende sekundäre Inhaltsstoffe der Lamiaceae. Neben Hydroxycinnamoylshikimat und -chinat, die im Pflanzenreich nahezu ubiquitär verbreitet sind, ist Rosmarinsäure hauptsächlich in den Boraginaceae und der Unterfamilie Nepetoideae der Lamiaceae zu finden. Die Biosynthes...

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Main Author: Sander, Marion
Contributors: Petersen, Maike (Prof. Dr.) (Thesis advisor)
Format: Dissertation
Published: Philipps-Universität Marburg 2010
Pharmazeutische Biologie
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44. Acyltransferasen erstellt. Die gefundenen Verwandtschaftsverhältnisse sind mit den von D´Auria (2006) beschriebenen Ergebnissen vergleichbar. Daher wurde die damals eingeführte Nummerierung der Klassen beibehalten. Als Basis für den Stammbaum dienten Proteinsequenzen aus der UniProt-Datenbank, deren Substratspezifität bekannt ist.

45. Abkürzungen Klasse I: At3AT1 Arabidopsis thaliana Cumaroyl-CoA:Cyanidin-3-O-glucose Hydroxycinnamoyltransferase; At3AT2 Arabidopsis thaliana Cumaroyl-CoA:Cyanidin-3-O-glucose Hydroxycinnamoyltransferase; MtMAT3 Medicago truncatula Isoflavon-7-O-glykosid Malonyltransferase; MtMAT1 Medicago truncatula Isoflavon-7-O-glykosid Malonyltransferase; At5MaT Arabidopsis thaliana Malonyl- CoA:Anthocyan-5-O-glucosid 6´´-O-Malonyltransferase; Dm3MAT2 Dendranthema x morifolium Anthocyanidin-3- O-glucosid 3´´,6´´-O-Dimalonyltransferase; Dm3MAT1 Dendranthema x morifolium Anthocyanidin-3-O-glucosid 6´´-O-Malonyltransferase; Sc3MaT Senecia curentus Malonyl-CoA:Anthocyanidin 3-O-glucosid 6´´-O- Malonyltransferase; Dv3MAT Dhalia variabilis Malonyl-CoA:Anthocyanidin-3-O-glucosid 6´´-O- Malonyltransferase; Dv3MAT2 Dahlia variabilis Malonyl-CoA:Anthocyanidin-3-O-glucosid 6´´-O- Malonyltransferase; Dm3MAT3 Dendranthemum x morifolium Anthocyanidin-3-O-glucosid 6´´-O- Malonyltransferase; Pf3AT Perilla frutescens Hydroxycinnamoyl-CoA:Anthocyanin-3-O-glucosid 6´´-O- Hydroxycinnamoyl-transferase; Ss3MAT Salvia splendens Anthocyanin-3-O-glucosid 6´´-O- Hydroxycinnamoyltransferase; Pf5MaT Perilla frutescens Anthocyanin-5-O-glucosid 6´´-O-Malonyltransferase; Ss5MaT Salvia splendens Malonyl-CoA:Anthocyanin-5-O-Glucosid 6´´´-O-Malonyltransferase; Gt5AT Gentiana triflora Hydroxycinnamoyl-CoA:Anthocyanin-5-O-glucosid 6´´´-O-Acyl-transferase; NtMAT1 Nicotiana tabacum Malonyl-CoA:Flavonoid/Naphtholglucosid Acyltransferase; Vh3MAT2 Verbena hybrida Quercetin-3-O-glucosid 6´´-O-Malonyltransferase; Vh3MAT1 Verbena hybrida Flavonol-3-O-glucosid 6´´-O-Malonyltransferase; Lp3MAT1 Lamium purpureum Flavonol-3-O-glucosid 6´´-O-Malonyltransferase; Klasse II: -Klasse III: CbBEAT Clarkia breweri Acetyl-CoA:Benzylalkohol Acetyltransferase; CcBEAT1-3 Clarkia concinna Acetyl-CoA:Benzylalkohol Acetyltransferasen; PsSalAT Papaver somniferum Salutaridinol 7-O-Acetyltransferase; CmAAT4 Cucumis melo Alkohol Acyltransferase; RhAAT1 Rosa hybrida Acetyl-CoA:Geraniol Acetyltransferase; SAAT Strawberry Alkohol Acyltransferase; VAAT Fragaria vesca Alkohol Acyltransferase; DAT Deacetylvindolin 4-O-Acetyltransferase; MAT Minovincinin 19-O-Acetyltransferase; RsVS Rauwolfia serpentina Vinorin-synthase; Ss5MaT2 Salvia splendens Malonyl-CoA:Anthocyanin-5-O-glucosid 6´´´-O-Malonyltransferase; Klasse IV: ACT Agmatin Cumaroyltransferase; Klasse Va: AtHHT1 Arabidopsis thaliana Hydroxycinnamoyl-CoA:ω-Hydroxysäure Hydroxycinnamoyl-transferase; DBBT Benzoyl-CoA:Taxan 2α-O-Benzoyltransferase; TAT Taxa-4(20),11(12)-dien- 5α-ol O-Acetyltransferase; DBAT 10-Deacetylbaccatin-III 10-O-Acetyltransferase; BAPT Baccatin III O- Phenylpropanoyltransferase; DBNTBT N-Debenzoyl-2´-deoxy-paclitaxel:N-Benzoyltransferase; AtSDT Arabidopsis thaliana Spermidin Disinapoyltransferase; AtSCT Arabidopsis thaliana Spermidin Dicumaroyltransferase; BanAAT Banana Alkohol Acyltransferase; CmAAT1 Cucumis melo Alkohol Acyltransferase; HMT/HLT Tigloyl-CoA:(-)-13α- hydroxymultiflorin/(+)-13α-hydroxylupanin O-Tigloyltransferase; CHAT (Z)-3-Hexen-1ol-O-Acetyltransferase; MpAAT1 Malus pumila Alkohol Acyltransferase; MdAAT2 Malus domestica Alkohol Acyltransferase; AMAT Anthraniloyl-CoA:Methanol Anthraniloyltransferase; CbBEBT Clarkia breweri Benzoyl-CoA:Benzylalkohol Benzoyltransferase; CmAAT3 Cucumis melo Alkohol Acyltransferase; NtBEBT Nicotiana tabacum Benzoyl- CoA:Benzylalkohol Benzoyltransferase; BPBT Benzoyl-CoA:benzylalkohol/phenylethalon Benzoyltransferase; Klasse Vb: CcHQT Cynara cardunculus Hydroxycinnamoyl-CoA:Chinat Hydroxycinnamoyltransferase; AtHCT Arabidopsis thaliana Hydroxycinnamoyl-CoA:Shikimat/Chinat Hydroxy-cinnamoyltransferase; TpHCT1 Trifolium pratense Hydroxycinnamoyl-CoA:Shikimat/Chinat Hydroxycinnamoyltransferase; TpHCT2 Trifolium pratense HydroxycinnamoylCoA:Maleinsäure Hydroxycinnamoyltransferase; NtHST Nicotiana tabacum Hydroxycinnamoyl- CoA:Shikimat Hydroxycinnamoyltransferase; CcHCT Coffea canephora Hydroxycinnamoyl-CoA:Shikimat/ Chinat Hydroxycinnamoyltransferase; CbHST Coleus blumei Hydroxycinnamoyl-CoA:Shikimat Hydroxycinnamoyltransferase; GhHST-k Glechoma hederacea Hydroxycinnamoyl-CoA:Shikimat Hydroxycinnamoyltransferase; GhHST-l Glechoma hederacea Hydroxycinnamoyl-CoA:Shikimat Hydroxycinnamoyltransferase; AsHHT1Avena sativa Hydroxycinnamoyl-CoA:Hydroxy-anthranilat N- Hydroxycinnamoyltransferase; NtHQT Nicotiana tabacum Hydroxycinnamoyl-CoA:Chinat Hydroxycinnamoyl- transferase; CsHQT1 Cynara scolymus Hydroxycinnamoyl-CoA:Chinat Hydroxycinnamoyltransferase; LjHQT Lonicera japonica Hydroxycinnamoyl-CoA:Chinat Hydroxycinnamoyltransferase; GhHQT-k Glechoma hederacea Hydroxycinnamoyltransferase ?; GhHQT-l Glechoma hederacea Hydroxycinnamoyltransferase?; CbRAS Coleus blumei Rosmarinsäuresynthase (Hydroxycinnamoyl-CoA-Hydroxyphenyllactat Hydroxycinnamoyltransferase); LaRAS Lavandula angustifolia Rosmarinsäuresynthase; MoRAS Melissa officinalis Rosmarinsäuresynthase; GhRAS-l Glechoma hederacea Rosmarinsäuresynthase; GhRAS-k Glechoma hederacea Rosmarin-säuresynthase Pseudogen?; HCBT Hydroxycinnamoyl/Benzoyl-CoA:Anthranilat N-Hydroxycinnamoyltransferase; AtSHT Arabidopsis thaliana Feruloyl-CoA:Spermidin Hydroxycinnamoyltransferase; Klasse VI: CFAT Coniferylalkohol Acetyltransferase Klasse VII: FsTRI10 Fusarium sporotichioides Trichothecen 3-O-Acetyltransferase; FgTRI101Fusarium graminearum Trichothecen 3-O-Acetyltransferase;

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