SELMA - das ERAD-ähnliche Präproteintranslokationssystem der zweiten Plastidenmembran von Phaeodactylum tricornutum

Diatomeen spielen bei der globalen Kohlenstofffixierung eine maßgebliche Rolle und stellen als Hauptbestandteil des Phytoplanktons einen Großteil der marinen Biomasse dar. Wie viele andere Algengruppen, aber auch beispielsweise humanpathogene Organismen, wie der Malariaerreger P. falciparum, sind Di...

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Main Author: Hempel, Franziska
Contributors: Maier, Uwe G. (Prof. Dr.) (Thesis advisor)
Format: Dissertation
Published: Philipps-Universität Marburg 2009
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37. Curriculum Vitae Persönliche Daten Franziska Hempel geb. am 14.08.1980 in Frankfurt am Main Promotion 01/2007-12/2009

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56. Abb. A-5: Kontrollexperimente zum Split-GFP-Interaktionssystem in P. tricornutum. A. In der Positiv-Kontrolle wurden die bZIP-Domänen der Transkriptionsfaktoren Fos und Jun aus Ratte (bFos, bJun) an die Split-GFP-Fragmente N-GFP und C-GFP fusioniert und im heterologen System P. tricornutum exprimiert. Beide Fragmente interagieren wie erwartet im Nukleus der Zelle, sichtbar durch GFP-Fluoreszenz. B. In der Negativ-Kontrolle wurde ein Konstrukt mit einer Deletion in der bZIP-Domäne des Fos-Proteins (∆179-193) eingesetzt. bJun und bFos∆ können nicht interagieren, und es wird wie erwartet keine GFP-Fluoreszenz detektiert. Die Split-GFP Konstrukte wurden nach Hu et al. konstruiert (Hu & Kerppola, 2003). Skala = 10 µm. PAF – Plastidenautofluoreszenz.

57. NR-Promotor: Intergenische Sequenz 5´5´des Nitratreduktase- Gens von P. tricornutum (Chromosom 20, 363018-363286) NR-Terminator: Intergenische Sequenz 3´3´des Nitratreduktase- Gens von P. tricornutum (Chromosom 20, 366341-36658)

58. Im Weitern gilt mein Dank Prof. Dr. Klaus Lingelbach für die Übernahme der Zweitkorrektur dieser Arbeit sowie Prof. Dr. Andreas Brune und Prof. Dr. Ralf Jacob als weiteren Mitgliedern der Prüfungskommission.

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