Immunhistochemische Identifikation und Differenzierung von Liposarkomen mittels verschiedener Fett-assoziierter Proteine

The present work occupies itself with the immunhistochemical reaction patterns of three new antibodies against lipid-associated proteins. Adipophilin (ADFP) is a protein, that in undifferentiated adipocytes, but, to find also in numerous other cell types. TIP 47 is a protein, that also found in different cells. Perilipin can be found only in adipocytes and steroidogenic cells. It was examined the occurrence and behavior of adipophilin, perilipin and tip47 in human normal tissues, in pathologically changed tissues, in liposarcomas and in other soft tissue sarcomas. The immunhistochemical examination took place with the ABC-technique at paraffinembedded material. Liposarcomas usually were most positive in different size, for adipophilin. Perilipin-positive were 70% of the liposarcomas and all others soft tissues sarcomas were negative. With the antibody agaimst tip 47 the reaction were different. For the first time, in the present study could be characterized three new lipid-associated antigens in liposarcomas and other soft tissues sarcomas.