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SufD - Bestandteil eines essentiellen plastidär lokalisierten Systems in Cyanobakterien, Cryptomonaden und höheren Pflanzen
Durch die vorliegende Arbeit konnte ein Beitrag zur funktionellen Charakterisierung des Nucleomorph-kodierten und plastidär lokalisierten Proteins ORF467 aus Guillardia theta geleistet werden. Dieses Protein konnte durch Sequenzhomologien als Bestandteil SufD des plastidären SUF-Systems identifizie...
| Prif Awdur: | |
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| Awduron Eraill: | |
| Fformat: | Dissertation |
| Iaith: | Almaeneg |
| Cyhoeddwyd: |
Philipps-Universität Marburg
2005
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| Mynediad Ar-lein: | Testun PDF llawn |
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The nucleomorph-encoded and plastid-locallized protein ORF467 of Guillardia theta has been identified as SufD, a component of the SUF-System in plastids. A gene for SufS was identified in the nucleus genome of Guillardia theta, indicating a unique genetic compartmentalization of the SUF-System in cryptophytes. The characterization of a SufD (ATNAP6) Arabidopsis thaliana T-DNA mutant revealed defects in embryo-development, reduced root-growth, altered chloroplast ultrastructure and reduced chlorophyll-content. A GFP-fusion protein of ATNAP6 was localized in plastidic spots, this localization pattern was caused by a beta-helix protein domain of ATNAP6. The complex phenotypes of the ATNAP6 mutation were interpreted as a defect of a plastidic system for iron-sulfur cluster biogenesis in Arabidopsis thaliana.