Die mitogen aktivierten Proteinkinasen ERK1 und ERK2 als Bestandteil der Mechanotransduktion in primären bovinen Osteoblasten

It is known that many cells are sensitive to mechanical loading. They respond to it by inducing and modulating biochemical pathways. One well established response of osteoblasts is to increase their metabolism and proliferation rate. In this work we investigated single, well known members of the signal transduction pathway such as activation of the proteins ERK1 and ERK2 in primary bovine osteoblasts after mechanical stress and unter microgravity conditions. The result shows a significant activation of ERK1 and ERK2 2-10 minutes post stretch and also a participation of these proteins in the microgravity signaltransduction. The results of this work could help to understand intracellular signalling pathways participating in mechanotransduction. Apart from this, information could be gained to develop new therapeutical drugs and training programs for patients suffering osteoporosis or other bone diseases.It is known that many cells are sensitive to mechanical loading. They respond to it by inducing and modulating biochemical pathways. One well established response of osteoblasts is to increase their metabolism and proliferation rate. In this work we investigated single, well known members of the signal transduction pathway such as activation of the proteins ERK1 and ERK2 in primary bovine osteoblasts after mechanical stress and unter microgravity conditions. The result shows a significant activation of ERK1 and ERK2 2-10 minutes post stretch and also a participation of these proteins in the microgravity signaltransduction. The results of this work could help to understand intracellular signalling pathways participating in mechanotransduction. Apart from this, information could be gained to develop new therapeutical drugs and training programs for patients suffering osteoporosis or other bone diseases.