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Identification of NAD-RNA species and ADPR-RNA decapping in Archaea
NAD is a coenzyme central tometabolism that also serves as a 5′-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model ar...
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| Autoren: | , , , , , , , , , |
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| 格式: | Artikel |
| 語言: | 英语 |
| 出版: |
Philipps-Universität Marburg
2024
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| 主題: | |
| 在線閱讀: | PDF-Volltext |
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| 總結: | NAD is a coenzyme central tometabolism that also serves as a 5′-terminal cap
for bacterial and eukaryotic transcripts. Thermal degradation of NAD can
generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and
NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model
archaeon Sulfolobus acidocaldarius and in the halophilic mesophile Haloferax
volcanii. None of the four Nudix proteins of S. acidocaldarius catalyze NADRNA
decapping in vitro, but one of the proteins (Saci_NudT5) promotes ADPRRNA
decapping. NAD-RNAs are converted into ADPR-RNAs, which we detect in
S. acidocaldarius total RNA. Deletion of the gene encoding the 5′−3′ exonuclease
Saci-aCPSF2 leads to a 4.5-fold increase in NAD-RNA levels.We propose
that the incorporation of NAD into RNA acts as a degradation marker for SaciaCPSF2.
In contrast, ADPR-RNA is processed by Saci_NudT5 into 5′-p-RNAs,
providing another layer of regulation for RNA turnover in archaeal cells. |
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| Item Description: | Gefördert durch den Open-Access-Publikationsfonds der UB Marburg. |
| DOI: | 10.1038/s41467-023-43377-x |