Software development for tracing QDs-labeled cells in coculture

As known, tracing cells of different types in coculture is very complicated, mainly because many cells have very similar morphology and it is difficult to distinguish the cells by morphology. Currently there is no simple assay which allows such measurements, since there is no easy way to automate qu...

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Bibliographische Detailangaben
1. Verfasser: Yang,Fang
Format: Arbeit
Sprache:Englisch
Veröffentlicht: Philipps-Universität Marburg 2010
Physik
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Zusammenfassung:As known, tracing cells of different types in coculture is very complicated, mainly because many cells have very similar morphology and it is difficult to distinguish the cells by morphology. Currently there is no simple assay which allows such measurements, since there is no easy way to automate quantitative distinction between the individual cell types. This is the reason why we decided to find a new method to realize automate this process. But the most important thing is to develop an assay for quantification of cell adhesion within cell-coculture, since there are obviously too many cells to count with hands and eyes.we find Igor Pro to count and analyze the images of labeled cells.