Publikationsserver der Universitätsbibliothek Marburg
Titel:Single-molecule Dynamics in Protein Interactions: Characterization of RarA and RecD2 of Bacillus subtilis
Autor:Romero Gonzalez, Hector
Weitere Beteiligte: Graumann, Peter L. (Prof. Dr.)
Veröffentlicht:2018
URI:https://archiv.ub.uni-marburg.de/diss/z2018/0110
URN: urn:nbn:de:hebis:04-z2018-01107
DOI: https://doi.org/10.17192/z2018.0110
DDC: Chemie
Titel (trans.):Einzelmoleküldynamik in Proteinwechselwirkungen: Charakterisierung von RarA und RecD2 von Bacillus subtilis
Publikationsdatum:2018-05-03
Lizenz:https://creativecommons.org/licenses/by-nc-sa/4.0

Dokument

Schlagwörter:
Fluoreszenzmikroskopie, single-molecule microscopy, Bakterien, Homologous recombination, Mikroskopische Technik, Molekulargenetik, Bacillus subtilis, DNA Reparatur, DNA repair, homologe Rekombination, fluorescence microscopy, Bacillus subtilis

Summary:
Maintenance of genome integrity is one of the crucial functions in life, to preserve the appropriate genetic information, being homologous recombination a key process in the DNA repair. I have used a novel technique, using slim-field microscopy to obtain single-molecule dynamics of two poorly described proteins, RarA and RecD2, in different recombination deficient mutants and conditions to characterize them. Single-molecule microscopy has been shown as a powerful method for in vivo characterization of proteins and its interactions. Together with genetics, I have added a new level of complexity in the regulation of homologous recombination as a multiway process in which many factors are involved in different avenues with partially overlapping functions depending on the kind of DNA damage generated. I have characterized RarA and RecD2 as factors involved in recombination, but also in replication of the DNA, being part of both RecA-independent and RecA-dependent replication progression, and antagonistic regulators of RecA filamentation. RarA plays its role in replication through interactions with DnaB, and in recombination as a RecA positive regulator through its interactions with RecA, RecO, RecR, RecD2 and RecU. RarA is regulated by the RecQ-like helicases RecQ and RecS. RecD2 plays a role in chromosomal segregation that becomes essential in the absence of RecG or RuvAB, and is a negative regulator in homologous recombination that interacts with RecA, RarA, RecX, RecF and PcrA.

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