Dokument

Summary:
The presented work describes the development of new ligand-binding assay platforms for rapid serological diagnosis. 3-D polyethylene sinter bodies and monodisperse gold nanoparticles have been successfully employed as protein carriers, providing sensitive, selective and rapid serological diagnosis. Detection of anti-Borrelia antibodies in human sera samples was taken as a model in our work. Antibodies recognition either by visible observation or by spectroscopic measurements was easily practicable. All developed assays could be performed in a short time, 5-30 min, providing a suitable colorimetric point of care test. The established assays offer simple, rapid and reliable tools of analysis with minimal cost, which can be easily transferred to other infectious diseases.

Bibliographie / References

1. Langmuir. 2008;24(22):12963-71. 128. Carrara S, Cavallini A, Maruyama Y, Charbon E, De Micheli G. A new ethylene glycol-silane monolayer for highly-specific DNA detection on silicon chips. Surface Science.
2. Applied and Environmental Microbiology. 2010;76(13):4143-50. 108. Kehrel M, Dassinger N, Merkl S, Vornicescu D, Keusgen M. A novel approach for increasing sensitivity in lateral flow assays: development of an enrichment module based on polyethylene sintered bodies. physica status solidi (a). 2012;209(5):917-24. 109. Bartczak D, Kanaras AG. Preparation of peptide-functionalized gold nanoparticles using one pot EDC/sulfo-NHS coupling. Langmuir. 2011;27(16):10119-23. 110. Hughes-Jones NC, Gardner B, Telford R. The effect of pH and ionic strength on the reaction between anti-D and erythrocytes. Immunology. 1964;7(1):72-81. 111. Barnes AE. The specificity of pH and ionic strength effects on the kinetics of the Rh (D)-anti-Rh (D) system. The Journal of Immunology. 1966;96(5):854-64. 112. Dumetz AC, Snellinger-O'Brien AM, Kaler EW, Lenhoff AM. Patterns of proteinprotein interactions in salt solutions and implications for protein crystallization. Protein Science : A Publication of the Protein Society. 2007;16(9):1867-77. 113. Peltomaa M, McHugh G, Steere AC. The VlsE (IR6) peptide ELISA in the serodiagnosis of Lyme facial paralysis. Otology & Neurotology. 2004;25(5). 114. Johnson BJB, Robbins KE, Bailey RE, Cao B-L, Sviat SL, Craven RB, et al.
3. Cellular and Molecular Life Sciences CMLS. 2001;58(5):794-800. 106. Höbel S, Vornicescu D, Bauer M, Fischer D, Keusgen M, Aigner A. A novel method for the assessment of targeted PEI-based nanoparticle binding based on a static surface plasmon resonance system. Analytical Chemistry. 2014;86(14):6827-35. 107. Brunt J, Webb MD, Peck MW. Rapid affinity immunochromatography column-based tests for sensitive detection of clostridium botulinum neurotoxins and Escherichia coli O157.
4. European Journal of Pharmaceutics and Biopharmaceutics. 2016;100:94-100. 34. Ramaraj T, Angel T, Dratz EA, Jesaitis AJ, Mumey B. Antigen-antibody interface properties: composition, residue interactions, and features of 53 non-redundant structures.
5. Journal of Clinical Microbiology. 2000;38(5):1735-9. 98. Porwancher R. A reanalysis of IgM western blot criteria for the diagnosis of early Lyme disease. Journal of Infectious Diseases. 1999;179(4):1021-4. 99. Ledue TB, Collins MF, Young J, Schriefer ME. Evaluation of the recombinant VlsEbased Liaison chemiluminescence immunoassay for detection of Borrelia burgdorferi and diagnosis of Lyme disease. Clinical and Vaccine Immunology : CVI. 2008;15(12):1796-804. 100. Boyd M, Woolley T. Point of care testing. Surgery (Oxford). 2016;34(2):91-3. 101. Dassinger N, Vornicescu D, Merkl S, Kehrel M, Dayyoub E, Bakowsky U, et al. A fusion protein for regenerative surfaces. Physica Status Solidi (a). 2012;209(5):832-8. 102. Miron T, Wilchek M. A simplified method for the preparation of succinimidyl carbonate polyethylene glycol for coupling to proteins. Bioconjugate Chemistry.
6. Bioconjugation and characterisation of gold colloid-labelled proteins. Journal of Immunological Methods. 2010;356(1-2):60-9. 105. Van Regenmortel MHV. Analysing structure-function relationships with biosensors.
7. Journal of Biological Chemistry. 1982;257(7):3669-75. 15. Woodhead JS, Weeks I. Chemiluminescence immunoassay. Pure and Applied Chemistry1985. p. 523. 16. Whitesides GM. The origins and the future of microfluidics. Nature.
8. 2007;68(2):218-24. 122. Dusemund B, Hoffmann A, Salzmann T, Kreibig U, Schmid G. Cluster matter: the transition of optical elastic scattering to regular reflection. Zeitschrift für Physik D Atoms, Molecules and Clusters.20(1):305-8. 123. Vilela D, González MC, Escarpa A. Sensing colorimetric approaches based on gold and silver nanoparticles aggregation: chemical creativity behind the assay. A review.
9. 1993;4(6):568-9. 103. Meyer MHF, Hartmann M, Krause H-J, Blankenstein G, Mueller-Chorus B, Oster J, et al. CRP determination based on a novel magnetic biosensor. Biosensors and Bioelectronics. 2007;22(6):973-9. 104. Thobhani S, Attree S, Boyd R, Kumarswami N, Noble J, Szymanski M, et al.
10. Development of an electrochemical ELISA for the screening of 17[small beta]-estradiol and application to bovine serum. Analyst. 2000;125(8):1419-23. 12. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry. 1971;8(9):871-4. 13. Van Weemen BK, Schuurs AHWM. Immunoassay using antigen-enzyme conjugates. FEBS Letters. 1971;15(3):232-6. 14. Josephy PD, Eling T, Mason RP. The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.
11. Development of an immunofiltration-based antigen-detection assay for rapid diagnosis of Ebola virus infection. Journal of Infectious Diseases. 2007;196(Supplement 2):S184-S92. 121. Meyer MHF, Stehr M, Bhuju S, Krause H-J, Hartmann M, Miethe P, et al. Magnetic biosensor for the detection of Yersinia pestis. Journal of Microbiological Methods.
12. 2016;905:140-8. 2. Imai S, Takahashi T, Naito S, Yamauchi A, Okada C, Notsu Y, et al. Development of a novel immunoassay specific for mouse intact proinsulin. Analytical Biochemistry.
13. 2015;407(13):3817-29. 78. De Jong WH, Borm PJA. Drug delivery and nanoparticles: applications and hazards.
14. Talanta. 2010;81(4-5):1560-7. 6. Glass TR, Saiki H, Joh T, Taemi Y, Ohmura N, Lackie SJ. Evaluation of a compact bench top immunoassay analyzer for automatic and near continuous monitoring of a sample for environmental contaminants. Biosensors and Bioelectronics. 2004;20(2):397-403. 7. Ma S, Tang Y, Liu J, Wu J. Visible paper chip immunoassay for rapid determination of bacteria in water distribution system. Talanta. 2014;120:135-40. 8. Banga-Mboko H, Sulon J, Closset J, Remy B, Youssao I, De Sousa NM, et al. An Improved Radioimmunoassay for Measurement of Pepsinogen in Porcine Blood Samples.
15. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 2012;1824(3):520-32. 35. Reverberi R, Reverberi L. Factors affecting the antigen-antibody reaction. Blood Transfusion. 2007;5(4):227-40. 36. Hnaien M, Diouani MF, Helali S, Hafaid I, Hassen WM, Renault NJ, et al.
16. Analytica Chimica Acta. 2012;751:24-43. 124. Kritskaya DA, Pomogailo AD, Ponomarev AN, Dyaschkovskii FS. Functionalization of polymer supports for polymerization catalysts by graft polymerization method. Journal of Applied Polymer Science. 1980;25(3):349-57. 125. Dowbenko R. Allyl monomers and polymers. Kirk-Othmer Encyclopedia of Chemical Technology: John Wiley & Sons, Inc.; 2000. 126. Bluemel J. Reactions of ethoxysilanes with eilica: a solid-state NMR study. Journal of the American Chemical Society. 1995;117(7):2112-3. 127. Pasternack RM, Rivillon Amy S, Chabal YJ. Attachment of 3- (aminopropyl)triethoxysilane on silicon oxide surfaces: dependence on solution temperature.
17. 2014;46:462-9. 5. Tang L, Dong C, Ren J. Highly sensitive homogenous immunoassay of cancer biomarker using silver nanoparticles enhanced fluorescence correlation spectroscopy.
18. 2015;484:91-8. 3. Yalow RS, Berson SA. Immunoassay of endogenous plasma in man. Journal of Clinical Investigation. 1960;39(7):1157-75. 4. Burmistrova NA, Rusanova TY, Yurasov NA, Goryacheva IY, De Saeger S. Multidetection of mycotoxins by membrane based flow-through immunoassay. Food Control.
19. Biosensors and Bioelectronics. 2007;22(7):1197-204. 19. Dzantiev BB, Byzova NA, Urusov AE, Zherdev AV. Immunochromatographic methods in food analysis. TrAC Trends in Analytical Chemistry. 2014;55:81-93. 20. Young S-H, Antonini JM, Roberts JR, Erdely AD, Zeidler-Erdely PC. Performance evaluation of cytometric bead assays for the measurement of lung cytokines in two rodent models. Journal of Immunological Methods. 2008;331(1-2):59-68. 21. Zhang Y, Li L, Lu J, Ge L, Ge S, Yan M, et al. Triple catalysis amplification strategy for simultaneous multiplexed electrochemical immunoassays based on cactus-like MnO2 functionalized nanoporous gold. Sensors and Actuators B: Chemical. 2013;186:545-9. 22. Wang X, Niessner R, Tang D, Knopp D. Nanoparticle-based immunosensors and immunoassays for aflatoxins. Analytica Chimica Acta. 23. Lin P, Wang S-H, Wang Y-L. Development of phase separation immunoassay.
20. 2012;73(3):243-5. 55. Barbour AG, Burgdorfer W, Hayes SF, Péter O, Aeschlimann A. Isolation of a cultivable spirochete fromIxodes ricinus ticks of Switzerland. Current Microbiology.
21. Chemical Reviews. 1957;57(4):665-742. 67. Domski GJ, Rose JM, Coates GW, Bolig AD, Brookhart M. Living alkene polymerization: new methods for the precision synthesis of polyolefins. Progress in Polymer Science. 2007;32(1):30-92. 68. Nakabayashi K, Mori H. Recent progress in controlled radical polymerization of Nvinyl monomers. European Polymer Journal. 2013;49(10):2808-38. 69. Pasanphan W, Chirachanchai S. Polyethylene film surface functionalized with chitosan via γ-ray irradiation in aqueous system: an approach to induce copper(II) ion adsorptivity on PE. Reactive and Functional Polymers. 2008;68(8):1231-8. 70. Patel D, Wu J, Chan P, Upreti S, Turcotte G, Ye T. Surface modification of low density polyethylene films by homogeneous catalytic ozonation. Chemical Engineering Research and Design. 2012;90(11):1800-6. 71. Daniloska V, Blazevska-Gilev J, Dimova V, Fajgar R, Tomovska R. UV light induced surface modification of HDPE films with bioactive compounds. Applied Surface Science.
22. Nature. 1997;390(6660):553-4. 51. Singh SK, Girschick HJ. Lyme borreliosis: from infection to autoimmunity. Clinical Microbiology and Infection. 2004;10(7):598-614. 52. Wagemakers A, Staarink PJ, Sprong H, Hovius JWR. Borrelia miyamotoi: a widespread tick-borne relapsing fever spirochete. Trends in Parasitology. 2015;31(6):260-9. 53. Singh SK, Girschick HJ. Toll-like receptors in Borrelia burgdorferi-induced inflammation. Clinical Microbiology and Infection. 2006;12(8):705-17. 54. Liveris D, Schwartz I, McKenna D, Nowakowski J, Nadelman R, DeMarco J, et al.
23. Multiplex immunoassay for Lyme disease using VlsE1-IgG and pepC10-IgM antibodies: improving test performance through bioinformatics. Clinical and Vaccine Immunology : CVI. 2011;18(5):851-9. 118. Gerritzen A, Brandt S. Serodiagnosis of Lyme borreliosis with bead based immunoassays using multiplex technology. Methods. 2012;56(4):477-83. 119. ABICAP-fast quantification of antigens and antibodies. Biosensors and Bioelectronics. 1993;8(2):xv-xviii. 120. Lucht A, Formenty P, Feldmann H, Götz M, Leroy E, Bataboukila P, et al.
24. 2016;80:249-56. 40. Zhang B, Zhang Y, Liang W, Cui B, Li J, Yu X, et al. Nanogold-penetrated poly(amidoamine) dendrimer for enzyme-free electrochemical immunoassay of cardiac biomarker using cathodic stripping voltammetric method. Analytica Chimica Acta.
25. Clinical Microbiology Reviews. 1997;10(1):185-201. 57. Brunner M. New method for detection of Borrelia burgdorferi antigen complexed to antibody in seronegative Lyme disease. Journal of Immunological Methods. 2001;249(1- 2):185-90. 58. Kelly R. Cultivation of Borrelia hermsi. Science. 1971;173(3995):443-4. 59. Molloy PJ, Persing DH, Berardi VP. False-positive results of PCR testing for Lyme disease. Clinical Infectious Diseases. 2001;33(3):412-3. 60. Branda JA, Aguero-Rosenfeld ME, Ferraro MJ, Johnson BJB, Wormser GP, Steere AC. 2-tiered antibody testing for early and late Lyme disease using only an immunoglobulin G blot with the addition of a VlsE band as the second-tier test. Clinical Infectious Diseases.
26. 2013;758:1-18. 25. Qi Y, Xiu F-R, Li B. One-step homogeneous non-stripping chemiluminescence metal immunoassay based on catalytic activity of gold nanoparticles. Analytical Biochemistry.
27. 1983;8(2):123-6. 56. Schmidt BL. PCR in laboratory diagnosis of human Borrelia burgdorferi infections.
28. Accounts of Chemical Research. 2000;33(1):27-36. 95. Lala N, Lalbegi SP, Adyanthaya SD, Sastry M. Phase transfer of aqueous gold colloidal particles capped with inclusion complexes of cyclodextrin and alkanethiol molecules into chloroform. Langmuir. 2001;17(12):3766-8. 96. Cevenini R, Sambri V, Massaria F, Franchini R, D'Antuono A, Borda G, et al.
29. Polymer Bulletin. 1996;37(1):89-96. 76. Rahimpour A, Madaeni SS, Zereshki S, Mansourpanah Y. Preparation and characterization of modified nano-porous PVDF membrane with high antifouling property using UV photo-grafting. Applied Surface Science. 2009;255(16):7455-61. 77. Vonk RJ, Wouters S, Barcaru A, Vivó-Truyols G, Eeltink S, Koning LJ, et al. Postpolymerization photografting on methacrylate-based monoliths for separation of intact proteins and protein digests with comprehensive two-dimensional liquid chromatography hyphenated with high-resolution mass spectrometry. Analytical and Bioanalytical Chemistry.
30. 2014;449:1-8. 26. Venn RF. Principles and practice of bioanalysis. London; New York: Taylor & Francis; 2000. 27. Kato K, Hamaguchi Y, Okawa S, Ishikawa E, Kobayashi K, Katunuma N. Enzyme immunoassay in rapid progress. The Lancet. 1977;309(8001):40. 28. Harlow E, Lane D. Antibodies : a laboratory manual. New York: Cold Spring Harbor Laboratory.; 1988. 29. Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F. Monoclonal versus polyclonal antibodies: distinguishing characteristics, applications, and information resources. ILAR Journal. 2005;46(3):258-68. 30. Bradley J. Immunoglobulins. Journal of Medical Genetics. 1974;11(1):80-90. 31. Bird RE, Hardman KD, Jacobson JW, Johnson S, Kaufman BM, Lee SM, et al.
31. 2008;39(3):443-9. 37. Shimabukuro FH, Da Costa VM, Da Silva RC, Langoni H, Da Silva AV, De Carvalho LR, et al. Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola. Memórias do Instituto Oswaldo Cruz. 2013;108(5):668-70. 38. De Azevedo PZ, Sylvestre TF, Cavalcante RdS, De Carvalho LR, Moris DV, De Oliveira MLCS, et al. Evaluation of the double agar gel immunodiffusion test and of the enzyme-linked immunosorbent assay in the diagnosis and follow-up of patients with chronic pulmonary aspergillosis. PLoS ONE. 2015;10(8):e0134841. 39. Lai W, Wei Q, Zhuang J, Lu M, Tang D. Fenton reaction-based colorimetric immunoassay for sensitive detection of brevetoxin B. Biosensors and Bioelectronics.
32. 2010;50(1):20-6. 61. prevention Cfdca. Readers recommendations for test performance and interpretation from the second national conference on serologic diagnosis of Lyme disease. MMWR Morb Mortal Wkly Rep. 1995;44(31):590-1. 62. Datta S, Christena LR, Rajaram YRS. Enzyme immobilization: an overview on techniques and support materials. 3 Biotech. 2012;3(1):1-9. 63. Chen W-J, Ling X-M. Advances of methods of protein immobilization on capillary column. Chinese Journal of Analytical Chemistry. 2009;37(6):929-34. 64. Sassolas A, Blum LJ, Leca-Bouvier BD. Immobilization strategies to develop enzymatic biosensors. Biotechnology Advances. 2012;30(3):489-511. 65. Mohamad NR, Marzuki NHC, Buang NA, Huyop F, Wahab RA. An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes. Biotechnology, Biotechnological Equipment. 2015;29(2):205-20. 66. Aggarwal SL, Sweeting OJ. Polyethylene: preparation, structure, and properties.
33. Chinese Journal of Analytical Chemistry. 2009;37(12):1839-46. 24. Pei X, Zhang B, Tang J, Liu B, Lai W, Tang D. Sandwich-type immunosensors and immunoassays exploiting nanostructure labels: a review. Analytica Chimica Acta.
34. Serodiagnosis of Lyme disease: accuracy of a two-step approach using a flagella-based ELISA and immunoblotting. Journal of Infectious Diseases. 1996;174(2):346-53. 115. Bacon RM, Biggerstaff BJ, Schriefer ME, Gilmore RD, Philipp MT, Steere AC, et al.
35. Serodiagnosis of Lyme disease by kinetic enzyme-linked immunosorbent assay using recombinant VlsE1 or peptide antigens of Borrelia burgdorferi compared with 2-tiered testing using whole-cell lysates. Journal of Infectious Diseases. 2003;187(8):1187-99. 116. Park S-H, Hwang K-J, Chu H, Park M-Y. Serological detection of Lyme borreliosis agents in patients from Korea, 2005-2009. Osong Public Health and Research Perspectives.
36. 2006;442(7101):368-73. 17. Giri B, Pandey B, Neupane B, Ligler FS. Signal amplification strategies for microfluidic immunoassays. TrAC Trends in Analytical Chemistry. 18. Lim CT, Zhang Y. Bead-based microfluidic immunoassays: the next generation.
37. The Veterinary Journal. 2003;165(3):288-95. 9. Larose P, Ong H, Du Souich P. Simple and rapid radioimmunoassay for the routine determination of vasopressin in plasma. Clinical Biochemistry. 1985;18(6):357-61. 10. Yalow RS. The limitations of radioimmunoassay (RIA). TrAC Trends in Analytical Chemistry. 1982;1(6):128-31. 11. Draisci R, Volpe G, Compagnone D, Purificato I, delli Quadri F, Palleschi G.
38. Single-chain antigen-binding proteins. Science. 1988;242(4877):423-6. 32. Schroeder Jr HW, Cavacini L. Structure and function of immunoglobulins. Journal of Allergy and Clinical Immunology. 2010;125(2, Supplement 2):S41-S52. 33. Higel F, Seidl A, Sörgel F, Friess W. N-glycosylation heterogeneity and the influence on structure, function and pharmacokinetics of monoclonal antibodies and Fc fusion proteins.
39. Surface immunofluorescence assay for diagnosis of Lyme disease. Journal of Clinical Microbiology. 1992;30(9):2456-61. 97. Magnarelli LA, Ijdo JW, Padula SJ, Flavell RA, Fikrig E. Serologic diagnosis of Lyme borreliosis by using enzyme-linked immunosorbent assays with recombinant antigens.
40. 2016;904:51-7. 41. Mazumdar SD, Barlen B, Kämpfer P, Keusgen M. Surface plasmon resonance (SPR) as a rapid tool for serotyping of Salmonella. Biosensors and Bioelectronics. 2010;25(5):967- 71. 42. Day MJ. Introduction to Antigen and Antibody Assays. Topics in Companion Animal Medicine. 43. Schnarr S, Franz JK, Krause A, Zeidler H. Lyme borreliosis. Best Practice & Research Clinical Rheumatology. 2006;20(6):1099-118. 44. Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwaldt E, Davis JP. Lyme disease-a tick-borne spirochetosis? Science. 1982;216(4552):1317-9. 45. Steere AC. Lyme borreliosis in 2005, 30 years after initial observations in Lyme Connecticut. Wien Klin Wochenschr. 2006;118(21-22):625-33. 46. Rosa PA, Tilly K, Stewart PE. The burgeoning molecular genetics of the Lyme disease spirochaete. Nat Rev Micro. 2005;3(2):129-43. 47. Charon NW, Goldstein SF. Genetics of motility and chemotaxis of a fascinating group of bacteria: the spirochetes. Annual Review of Genetics. 2002;36(1):47-73. 48. Pfister HW, Wilske B, Weber K. Lyme borreliosis: basic science and clinical aspects.
41. International Journal of Nanomedicine. 2008;3(2):133-49. 91. Shang Y, Min C, Hu J, Wang T, Liu H, Hu Y. Synthesis of gold nanoparticles by reduction of HAuCl4 under UV irradiation. Solid State Sciences. 2013;15:17-23. 92. Muddineti OS, Ghosh B, Biswas S. Current trends in using polymer coated gold nanoparticles for cancer therapy. International Journal of Pharmaceutics. 2015;484(1-2):252- 67. 93. Weiser HB. Inorganic colloid chemistry. 1933. 94. Templeton AC, Wuelfing WP, Murray RW. Monolayer-protected cluster molecules.
42. The Lancet. 1994;343(8904):1013-6. 49. Humair P-F, Gern L. The wild hidden face of Lyme borreliosis in Europe. Microbes and Infection. 2000;2(8):915-22. 50. Barbour AG, Zuckert WR. Genome sequencing: new tricks of tick-borne pathogen.
43. 2010;256(7):2276-83. 72. Price GJ, Clifton AA, Keen F. Ultrasonically enhanced persulfate oxidation of polyethylene surfaces. Polymer. 1996;37(26):5825-9. 73. Wickson BM, Brash JL. Surface hydroxylation of polyethylene by plasma polymerization of allyl alcohol and subsequent silylation. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 1999;156(1-3):201-13. 74. Austin ME, Busfield WK, Pomery PJ. Radiation-induced grafting of allyl alcohol onto oriented high density polyethylene. European Polymer Journal. 1995;31(7):683-7. 75. Yang WT, Rånby B. The role of far UV radiation in the photografting process.

Das Dokument ist im Internet frei zugänglich - Hinweise zu den Nutzungsrechten