Dokument
| Titel: | Towards Bioorthogonal Enzymatic Uncaging Reactions |
| Autor: | Ritter, Cornelia |
| Weitere Beteiligte: | Meggers, Eric (Prof. Dr.) |
| Veröffentlicht: | 2016 |
| URI: | https://archiv.ub.uni-marburg.de/diss/z2016/0051 |
| DOI: | https://doi.org/10.17192/z2016.0051 |
| URN: | urn:nbn:de:hebis:04-z2016-00513 |
| DDC: | Biowissenschaften, Biologie |
| Titel (trans.): | Entwicklung Bioorthogonaler Enzymatischer Demaskierungs-Reaktionen |
| Publikationsdatum: | 2016-01-25 |
| Lizenz: | https://creativecommons.org/licenses/by-nc-sa/4.0 |
| Schlagwörter: |
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| caging, Enzym, Schutzgruppe, Chemische Biologie, P450, P450, Aktivierung, Bioorthogonal, enzyme, chemical biology, activation |
Summary:
In this thesis, engineered cytochrome P450 monooxygenase mutants are reported as highly active and selective catalysts for the bioorthogonal uncaging of diferent ether protected substrates, including the successful transfer of the uncaging reaction into living bacteria. This proof-of-principle study points towards the utility of bioorthogonal enzyme/protecting group pairs for applications in the life sciences.
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