Dokument

Zusammenfassung:
Durch die Klonierung und rekombinante Expression verschiedener Pathogenitätsgene aus Shigella flexneri in Escherichia coli sowie die Aufreinigung der resultierenden Genprodukte konnten verschiedene biochemische Untersuchungen und Kristallisationsversuche an IpgA, IpgB, IpgC, IpgD, OSpD1, Spa15, IpaA, IpaB, IpaC und weitern durchgeführt werden. Diese Produkte sind Teil des Typ III-Translokationssystems und wichtig für die Invasion von Shigella in die Epithelzellen des Darms.

Bibliographie / References

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15. E R K L Ä R U N G Ich versichere, dass ich meine Dissertation " Klonierung und Expression von Shigella- Pathogenitätsgenen sowie Interaktionsstudien mit ihren Produkten " selbständig ohne unerlaubte Hilfe angefertigt und mich dabei keiner anderen als der von mir ausdrücklich bezeichneten Quellen bedient habe.
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27. IpgD330-530 gD330_f, gD-8_b BamHI/NotI IpgD∆1-329∆531-538
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31. OspD1-15,-4 D1-15_f, D1-4_b NdeI/XhoI OspD1∆1-15∆222-225
32. OspD1-80,-4 D1-80_f, D1-4_b NdeI/XhoI OspD1∆1-80∆222-225
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