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Titel:Crosslinking studies of the bacterial RNase P holoenzyme and functional characterisation of the novel protein-only RNase P from Arabidopsis thaliana
Autor:Pavlova, Liudmila Viktorovna
Weitere Beteiligte: Hartmann, Roland (Prof. Dr.)
Veröffentlicht:2012
URI:https://archiv.ub.uni-marburg.de/diss/z2012/0778
DOI: https://doi.org/10.17192/z2012.0778
URN: urn:nbn:de:hebis:04-z2012-07782
DDC: Naturwissenschaften
Titel (trans.):Crosslinking-Studien an bakteriellem RNase P Holoenzym und funktionelle Untersuchungen der kürzlich entdeckten Protein-Enzyme der RNase P von Arabidopsis thaliana
Publikationsdatum:2012-09-14
Lizenz:https://rightsstatements.org/vocab/InC-NC/1.0/

Dokument

Schlagwörter:
Kinetics, Crosslinking, Proteinaceous RNase P, Catalytical mechanism, Endoribonuklease P

Summary:
Ribonuclease P (RNase P) is the ubiquitous processing enzyme that generates the mature 5'-termini of tRNA molecules. In Bacteria and Archaea the enzyme is a ribonucleoprotein, whereas in some Eukarya the RNAse P is exclusively proteinaceous. This work is focused on two topics: (i) crosslinking studies of bacterial RNase P holoenzyme utilizing novel 2’- ribooligonucleotides and (ii) functional investigations to the catalytical mechanism of recently discovered protein-only RNase P from Arabidopsis thaliana.

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