Polyethylenimine and its derivates: investigation of in vivo fate, subcellular trafficking and development of novel vector systems

In this dissertation several aspects of polymer based gene delivery were investigated. First, key issues in subcellular processing of electrostatic polymer/nucleic acid complexes were investigated and new insights into mechanisms involved in these processes were gained. Secondly, a...

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Bibliographische Detailangaben
1. Verfasser: Merdan, Thomas (BetreuerIn (Doktorarbeit))
Format: Dissertation
Sprache:Englisch
Veröffentlicht: Philipps-Universität Marburg 2003
Pharmazeutische Technologie und Biopharmazie
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Zusammenfassung:In this dissertation several aspects of polymer based gene delivery were investigated. First, key issues in subcellular processing of electrostatic polymer/nucleic acid complexes were investigated and new insights into mechanisms involved in these processes were gained. Secondly, a targeted gene delivery system was developed for the specific transfection of ovarian carcinoma cells. The resulting vector exhibited a high specificity for target cells combined with low unspecific transfection and toxicity. Furthermore, a novel type of gene delivery system was synthesized. This vector exhibited a high in vitro transfection efficiency and a very low in vitro toxicity as well as favourable in vivo properties, such as reduced toxicities. Another aspect that was studied in depth was the investigation of the stability of several electrostatic vectors in vitro and when applied intravenously.